Distributed via the CDC Health Alert Network
June 21, 2016, 1140 EDT (11:40 AM EDT)
CDC Recommendations for Subsequent Zika IgM Antibody Testing
Summary Testing for Zika virus infection using real-time reverse-transcription polymerase chain reaction (rRT-PCR) molecular assays is now commercially available. When requesting Zika rRT-PCR testing from a commercial laboratory, providers should be aware that commercial laboratories performing rRT-PCR currently do not also offer Zika IgM enzyme-linked immunosorbent assay (ELISA) or confirmatory serologic testing (plaque reduction neutralization test, or PRNT). Therefore, if possible, providers should store a serum aliquot for subsequent Zika IgM ELISA testing if the rRT-PCR assay is negative. Otherwise, collection of an additional serum sample may be necessary.
rRT-PCR (molecular) testing should be performed for patients possibly exposed to Zika virus who have symptoms consistent with Zika virus infection
Providers who request molecular testing for Zika virus infection from a commercial testing laboratory are advised to retain and store in a refrigerator (2-8°C) an aliquot of the patient’s serum for subsequent Zika IgM ELISA testing if the rRT-PCR is negative
For specimens that are rRT-PCR negative from the commercial laboratory and no stored serum specimen is available, another serum specimen should be collectedwithin 12 weeks of symptom onset for Zika IgM ELISA testing
Appropriate samples for molecular testing are serum samples collected
Molecular assays for detection of Zika virus RNA are now commercially available under Emergency Use Authorizations (EUAs) issued by the Food and Drug Administration (FDA). CDC recommends molecular testing using rRT-PCR for serum samples collected
Currently, commercial laboratories that offer rRT-PCR testing do not provide Zika IgM ELISA testing with PRNT confirmation and have no routine process to forward specimens to another testing laboratory. Therefore, when requesting Zika rRT-PCR testing from a commercial laboratory, providers should retain an aliquot of the serum for Zika IgM ELISA testing if the rRT-PCR testing is negative. Blood should be collected and processed per routine guidelines (collected in a serum separator tube with serum aliquots transferred to new vials), and one of the serum aliquots should be stored in a refrigerator (2-8°Cntil it is known if additional IgM testing is indicated. If a serum aliquot cannot be stored or is not available, but further testing is indicated, a new blood sample should be collected. Serum samples for IgM testing should be collected from patients within 12 weeks of symptom onset. Providers should contact their local health department to discuss IgM testing of stored or newly collected serum from patients who are rRT-PCR negative.
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